Application not executing when pendrive removed

Hi all…i got an application exe in a pen drive. I tried to copy in to my desktop removed ther pendrive and running the application, it is throwing user defined message…but when the pendrive is attached from where i copied i was able to run from the copied desktop location.
I tried cloning of pendrive to other pendrive and it is not working. Can some one help me out to resolve this…

If the application you are referring to is SecureAccess it will only work when it is executed from the flash drive.

Thanks for the update ed.
I have one more clarification…whether it is possible to clone the secure access can i overcome this issue

It will only run on SanDisk drives.